Tissue culture involves growing plants from very small sections (sometimes microscopic) in a laboratory. It is a propagation method which is being increasingly used. Tissue culture is not appropriate for many plants, but for others such as orchids, some indoor plants and in particular, many new plant varieties, it is a very popular propagation method.
- Grow Ferns, Orchids, Cut Flowers or Other Plants.
- Learn to produce large quantities of new varieties fast and at low cost.
- Online or Correspondence
- Payment Plans available
- Unlimited tutor support by email.
Who is this course suitable for?
This course is suitable for anyone already working with plants and those who just have a specific interest in plant propagation either for a hobby or commercial purposes. The course will suit students who are looking for self-paced study and don't require face-to-face support.
Why use tissue culture for propagation of plants?
Plant tissue culture allows you to very quickly reproduce plants without requiring much space. One of the main benefits of this is speed to market with large quantities of plants or even new cultivars.
- Explain the nature of plant growth processes, in the tissue culture environment.
- Determine growing media to use for tissue culture.
- Specify appropriate micropropagation procedures for different purposes.
- Explain the management of environmental control equipment used in tissue culture.
- Design a layout for a commercial tissue culture facility.
- Determine appropriate commercial applications for tissue culture.
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Detailed Course Outline
This course is made up of a number of lessons or units. Each of these has self assessment questions, a set task (practical homework) and an assignment which you can upload online. Click below to read about each of the lessons and what you will learn.
There are 8 lessons in this course:
- The principles of propagating plants by cuttings.:Importance of cuttings
- Phenotype vs genotype
- Why choose cutting propagation
- Where to get cuttings from
- Basic cutting technique.
2. Stem cuttings
- Ease with which tissue forms roots
- Types of stem cuttings (softwood, hardwood, semi hardwood, herbaceous, tip, heel, nodal, cane etc)
- Treatments (eg. basal heat, mist, tent, etc)
- Testing rooting, etc.
3. Non-stem cuttings
- Leaf cuttings
- Root cuttings (natural suckering with or without division, Induced suckering, In situ whole root cuttings; ex situ detached root cuttings)
- Bulb cuttings, scaling and twin scaling, sectioning, basal cuttage.
4. Materials and Equipment
- Stock plant Quality
- Criteria for Selecting Plant Material
- Planting Out Stock Plants
- Care of Stock Plants
- Stock Plants for Root Cuttings
- Disinfecting cutting material
- Sources of Hypochorite
- Plant Containers
- Tools and Equipment
5. Growing Media
- Propagation media
- Biological, chemical and physical characteristics of propagation and potting media
- Testing for toxins
- Air filled porosity
- Nutrition Management
- Potting up cuttings
- Soil-less mixes, rockwool, etc.
6. Factors affecting Rooting
- Cutting Treatments
- Hormones & their application; auxins, cytokinins, gibberelins
- Applying Hormone
- Anti transparents, acid/base treatments, disinfectants etc
- Carbon Dioxide Enrichment, etc.
7. Setting up a Propagation Area
- Creating and managing an appropriate cutting environment in terms of: Water; Disease; Temperature; Light and Air Quality.
- Greenhouses and other structures, cloches, cold frames, greenhouses, etc
- Watering methods (mist, fog, capillary etc)
- Heating, etc.
- The Nursery Site
8. Management of Cutting Crops
- Estimating cost of production
- Efficiencies in Cutting Propagation
- Keeping records
When you have completed the lessons of your Certificate course, you will be given the option of taking the optional exam. It's okay if you don't want the exam, we still issue your Careerline Certificate. For Advanced Certificates however, the exam is compulsory (per module) and are included in the course fee.
Barbara Tremain BSc. (Hons) Horticulture, RHS Adv. Cert,. RHS General, City & Guilds Garden Design, Permaculture Level 3 (UK)
Barbara brings to Careerline a wide range of horticultural experience and knowledge Barbara has a keen interest in herbalism, edible wild plants and has lead into studies of many plant based complementary medicinal systems, qualification as a Bach Flower remedy practitioner and studying Ethnobotany. Barbara has practiced and studied horticulture as well as combining it with her love of travel in many countries including the UK, US and Australia and has also studied Permaculture and runs her own horticulture business. Within the past two years she have also worked for Groundwork SW with 18-24 year old unemployed people, this work and the mentoring scheme has allowed me to teach informally. It has been a delight to nurture the interests of keen learners in horticulture and garden design. Meanwhile the business has covered a variety of Garden Design projects, from wildflower gardens to seaside situations, and has included some work with Chelsea Gold multi-winner John Moreland.
Frequently Asked Questions
Can I pay in installments?
Yes! We offer great payment plans, however full payment up front is the cheapest. You can choose the best payment plan for you from the course page, or call us on 07 55368782 to talk to one of our course consultants for a custom plan.
Can I study from anywhere in the world?
We have many international students. Careerline's range of courses are suitable for any one, anywhere in the world.
Do I get a discount if I enroll in a second course?
Yes. You may claim a 5% fee discount when you enroll in a second course, and a further 10% off a three-course package.
Do I have to sit an Exam?
No. If you are enrolled in a Certificate course (100hrs), the exam is optional. You will be issued with a certificate which proves that you are competent in all units, if you choose not to sit the exam.
Do you have set start dates?
There are no set start dates, you may start at any time. Our courses are all self-paced. As our home page says, ‘Courses for Anyone, Anywhere, Anytime’. ‘Anytime’ includes the start time of your choice. However, we do encourage our students to submit assignments on a regular basis. Wherever possible, we suggest developing a study routine.